The ability to overexpress genes in microorganisms has revolutionized the field of synthetic biology. Gene overexpression involves the production of large quantities of target proteins by increasing the transcription and translation of the gene of interest. This technique has been applied in the engineering of microorganisms to produce a wide range of products, including biofuels, chemicals, and biopharmaceuticals.
There are several strategies for gene overexpression in strain engineering. One common method is gene synthesis, which involves the design and synthesis of genes optimized for expression in specific host organisms. Gene experts modify the codon usage, remove destabilizing sequences, and add regulatory elements to enhance expression. Therefore, the target expression level of the gene is achieved through the modification and design of the gene.
CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas. CD Biosynsis is fully engaged in providing customers around the world with satisfactory services and cost-effective solutions to promote the development of synthetic biology. Our scientific team is committed to helping our customers solve any problems encountered in their projects and bring diverse creative ideas to fruition.
Another approach is cloning. Experts utilize state-of-the-art techniques, such as restriction enzyme digestion and DNA ligation, polymerase chain reaction (PCR) amplification, plasmid-mediated transformation, Bacterial Strain Engineering and gene transfection, to clone synthesized genes into different expression vectors, enabling high levels of gene expression.
In some cases, to enhance gene expression, regulatory elements that may reduce gene expression levels can be identified and removed. This type of gene optimization can be achieved by modifying the promoter, ribosome binding site, and other regulatory elements to obtain optimal gene expression levels.
Overall, gene overexpression is a powerful tool in strain engineering to enhance the production of specific compounds or metabolic activities. It involves increasing the expression level of specific genes through techniques such as gene synthesis, cloning, gene optimization, and protein expression. From a synthetic biology perspective, researchers can explore the role of gene overexpression in strain engineering and how they can optimize gene overexpression for diverse applications.
Synthetic biology is an emerging interdisciplinary field that combines engineering principles and biology to create artificial life or redesign organisms for useful purposes. With a dedicated team and experience accumulated over the years, we can provide products and services that cover a wide range of steps in the synthetic biology workflow. CD Biosynsis offers comprehensive synthetic biology toolboxes and fully integrated platforms to tailor solutions according to customer’s needs. We have the expertise to support and facilitate the applications of synthetic biology in a wide range of areas, from basic life sciences research to biosensors.